Journal: PLoS ONE

Article Title: Induction of Type I and Type III Interferons by Borrelia burgdorferi Correlates with Pathogenesis and Requires Linear Plasmid 36

doi: 10.1371/journal.pone.0100174

Figure Lengend Snippet: Human PBMCs (5×10 6 ) were co-incubated for 12 hours with 5×10 7 B31-A3 or A3-M9 lp36−. Equal amounts of PBMC total RNA from each of three biological replicates was pooled, converted to cDNA and used as a template for the Human NF-κB Signaling Pathway RT 2 Profiler PCR Array. Genes that were transcriptionally induced or repressed ≥4-fold are indicated by red or green circles, respectively. Scatter plots depict transcriptional regulation in PBMCs stimulated with B31-A3 ( A ) or A3-M9 lp36− ( B ) versus unstimulated PBMCs. C , Comparison of transcript profiles of PBMCs stimulated with either B31-A3 or A3-M9 lp36− B. burgdorferi .

Article Snippet: Transcript levels were quantified based on the 2 −ΔΔCT method through the measurement of SYBR green fluorescence using the RT 2 Real-Time SYBR Green/Rox PCR Master Mix (PA-012; SABiosciences).

Techniques: Incubation